Fig. 2
One-round ePCR does not suppress formation of the chimeric molecules from two-plasmid template. a Agarose gel electrophoresis analysis of products of one-round ePCR generated using either the indicated amounts of an equimolar mixture of plasmid#1 and plasmid#2 (lanes 1–2) or water (no template control, “NTC”; lane 3) as template and primers Libr-A1-for/Libr-rev. b Agarose gel electrophoresis analysis of products of test PCR set up using either 1/100th of the purified “1 ng” one-round ePCR sample (lanes 1–4) or water (no template controls, “NTCs”; lanes 5–8) as template and the following primers: BC1/ROI1 (lanes 1 and 5), BC2/ROI2 (lanes 2 and 6), BC1/ROI2 (lanes 3 and 7) and BC2/ROI1 (lanes 4 and 8). c The principle of the water-in-oil emulsion stability assay. Emulsion PCR is set up using a mixture of two emulsions that contain micelles lacking primers or template DNA. In stable emulsions, micelles do not merge together that results in no amplification products. d Agarose gel electrophoresis analysis of ePCR products generated using either a mix of micelles lacking primers Libr-A1-for/Libr-rev or plasmid#1 DNA (lane 1) or micelles with all the reaction components (positive control, “C+”; lane 2) or micelles with water instead of the template DNA (no template control, “NTC”; lane 3). M stands for GeneRuler 1 kb Plus DNA Ladder (Thermo Fisher Scientific) in (a, b, d)