Fig. 4

CFTR translation is predicted to occur via non-uniform velocity, with synonymous mutations locally governing the rate of synthesis. a Computed translation rate of CFTR for various tested cell or tissue models. The gray area marks the 10th–90th percentile to emphasize slow and rapidly translated regions. Single CFTR domains are designated as color-coded bars at the top of the sequence: membrane-spanning domains 1 and 2 (MSD1, MSD2) (blue); nucleotide-binding domains 1 and 2 (NBD1, NBD2) (blue); regulatory (R)-domain (red). sSNPs with prevalence higher than 1% in CFTR are marked with (●), and the nucleotide exchange at the corresponding position is designated using the scheme: wild-type nucleotide, position, mutated nucleotide. b-d tRNA concentration within the tRNA^Thr (b), tRNA^Glu (c) and tRNA^Pro (d) families compared to those present in CFBE41o⁻ cells. The synonymous SNP-induced codon is designated in red. For iPSCs, only the zero time point was reported in all panels. tRNA isoacceptors are depicted with their cognate codon and the corresponding amino acid