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Table 2 Primers used for gene expression studies by qPCR analysis

From: The initiation of puberty in Atlantic salmon brings about large changes in testicular gene expression that are modulated by the energy status

Gene description Target gene Primers Primer sequence (5′ → 3′)
Anti-Müllerian hormone amh Fw CAGTCACTCTCTGCAGCCTTACAA
Rv CAACATTGAATCTCCATTTCAGTTTAC
Probe TTTGCCCTCGGGTTGCTTTCCTGTT
Calreticulin precursor calr Fw ACGCCGCTGACTCTACCATCT
Rv TCACATCATCTGAGACCAGGAAGTT
Catenin beta-1 ctnnb1 Fw CCCCAGGCGACAGCAAT
Rv CCAAATCACAATGCAGGTTGA
Cryptochrome-1 cry1 Fw AGGTCAGCCGGCCAACA
Rv AACGGCTCAGAGTCACACTCAAA
Endoplasmin precursor hsp90b1 Fw TGAAGCGGATTTGGTTTATTGG
Rv CGCCGTCAATGTCAAGTTCAT
Eukaryotic 18S ribosomal RNA 18S TaqMana proprietary information
Follicle stimulating hormone subunit beta fshb Fw TCACGGAGGCATCACCATCA
Rv GCTCTTGGCAACGGGTATGA
Probe ACCTGCGCCGGCCTGTGC
Frizzled-8 precursor fzd8l Fw GGAGCGACCCATTATTTTCCT
Rv CCGGCAATTAGTCTGACAATGTAA
G1/S-specific cyclin-D1 ccnd1 Fw AAGCAAATCCTGTGCAAGCA
Rv CGGAGGGTTGGCAATGAA
Gamma-crystallin M2 crygm2 Fw CCACCTTCTACGAGGACAGGAA
Rv TGGCACCTGCTCATGTAGGA
Gap junction alpha-1 protein gja1 Fw CCTAAGGGCTCTCTTCTTACTTCTGA
Rv CACTCCAGTCACCCATGATGAA
Geminin gmnn Fw TGCAAGTCCTCCAGCAAGCT
Rv CCCTTGTTCTGCATTCCACTGT
Gonadotropin releasing hormone receptor 4 gnrhr4 Fw TCAACCCACTGGCGATCAAT
Rv CGTGATGGTCACACTGTGGAATA
Probe AGTGTGATTCTGTCTGTTCCCCAGATGCTG
Gonadotropin subunit beta-2 lhb Fw ACACTGCCCATCAGGACACAA
Rv GGGAGATCAAGGTGCCTACATG
Growth-regulated alpha protein-like LOC106577834 Fw TCATCATGAATACTGCAATGACTGTT
Rv TATCCTCTCCCTTGTATCATGCAGAT
Insulin-like 3 (Leydig cell) insl3 Fw CTCCGGAGCTTGGACAACAC
Rv AGTCCTCAGGTTGGCAAATTGAT
Insulin-like growth factor 3 igf3 Fw GACCGACCGACAAGATGCA
Rv TGCAAGGCACAATATGGAGTACA
Noggin nog Fw GATGGAGGTGCCTGCAGAGA
Rv TTCAGTTCGAGCAGGAGCATTT
Olfactomedin-like protein 2B precursor olfml2b Fw CGAAGGGCAACGGAAATGTA
Rv ATCCAGTTGTACGGCAGCTTGT
Prostaglandin E synthase 3 ptges3 Fw CCGTGGCTGAGGCTAACAAA
Rv CCTCCCAGTCTTTCCAGTTGTTAA
Testin tes Fw CGTGTCTGAAATGCAAGGACAA
Rv GTGGTCCGTAAGCCCACACTT
Vasotocin-neurophysin VT 2 precursor neu4 Fw GGACACTGCGCTGCAACA
Rv GTACCGACTCTCCCCCAAACA
Wnt-11 precursor wnt11l Fw CCCCAGGCCAGCACACTA
Rv ACCACCTGCAGAGCCTCACT
  1. a20X eukaryotic 18S rRNA pre-developed TaqMan assay (4352930E; Applied Biosystems). Fw Forward, Rv Reverse