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Table 1 Statistical summary of RNA-Seq on Echiniscoides cf. sigismundi and Richtersius cf. coronifer (data from BGI)

From: Comparative transcriptomics suggest unique molecular adaptations within tardigrade lineages

 

Echiniscoides cf. sigismundi

Richtersius cf. coronifer

Total Bases

5,531,945,800

5,761,908,600

Number of raw reads

58,601,018

64,000,000

Number of clean reads

55,319,458

57,619,086

GC%

34.73%

47.40%

Q20

97.54%

96.63%

Number of contigs

55,499

84,106

Mean length of contigs (bp)

450

454

N50contigs

1176

1259

Number of Unigenes

31,601

55,053

Mean length of Unigenes

830

1052

N50Unigenes

1524

2197

Number of Unigenes > 1 kb

9974

20,319

Number of Unigenes > 3 kb

1068

4589

FPKMmean / (min-max)

27.3587/(0.0–44,747.82)

13.4950/(0.0–14,668.10)

FPKMmedian

4.6156

2.1674

FPKMsum

864,563.2158

742,939.1452

TPMmean / (min-max)

31.6446 (0–51,757.72)

18.1643 (0–19,743.35)

TPMmedian

5.3387

2.9173

Number of Unigenes after filteringa

15,784

21,384

Mean length of Unigenes after filtering

1175

1160

  1. aFor the detailed analysis of stress related genes (see Table 3), we applied the following filtering parameters: FPKM > 1; transcript length > 300 bp; longest contig for each locus (see text for details)