Table 4 Oligonucleotides used in 3’RACE experiments
General primers | Primer sequence | Step | ||
GR3-dT | 5′-GCTGTCAACGATACGCTACGTAACGGCATGACAGTG(t)18(g,c,a)-3’ | RT | ||
GR3–1 | 5′-GCTGTCAACGATACGCTACGTAACG-3’ | First round PCR | ||
GR3–2 | 5′-CGCTACGTAACGGCATGACAGTG-3’ | Second round PCR | ||
Gene specific primers | Primer sequence | PolyA site tested | Position (nt after stop) | Step |
Olfr1508–1 | 5′-AGCAGCAAACATTCCAAATTGAGGA-3’ | M | 1001 | First round PCR |
Olfr1508–2 | 5′-TGTGTTCACCTAAAGAGTTTAAGAC-3’ | 1145 | Second round PCR | |