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Table 4 Oligonucleotides used in 3’RACE experiments

From: Alternative polyadenylation produces multiple 3’ untranslated regions of odorant receptor mRNAs in mouse olfactory sensory neurons

General primers Primer sequence Step
 GR3-dT 5′-GCTGTCAACGATACGCTACGTAACGGCATGACAGTG(t)18(g,c,a)-3’ RT
 GR3–1 5′-GCTGTCAACGATACGCTACGTAACG-3’ First round PCR
 GR3–2 5′-CGCTACGTAACGGCATGACAGTG-3’ Second round PCR
Gene specific primers Primer sequence PolyA site tested Position (nt after stop) Step
 Olfr1508–1 5′-AGCAGCAAACATTCCAAATTGAGGA-3’ M 1001 First round PCR
 Olfr1508–2 5′-TGTGTTCACCTAAAGAGTTTAAGAC-3’ 1145 Second round PCR