Fig. 7

Analysis of the relative expression of Podosphaera xanthii genes coding for haustorium-specific secreted proteins during the first steps of infection. a Relative expression of genes analysed by quantitative reverse transcription–polymerase chain reaction (qRT-PCR). Total RNA was isolated from zucchini cotyledons inoculated with P. xanthii at different time points (24, 48 and 72 h post-inoculation), and the relative expression of genes was analysed by qRT-PCR. Transcript abundance was normalized to the transcription of the endogenous control elongation factor-1 gene PxEF1 (MK249653). The data shown represent average values of three experimental replicates from three independent experiments, with error bars showing the standard error. b Hierarchical clustering of the relative expression of genes coding for haustorium-specific secreted proteins at different time points after inoculation (24, 48 and 72 h) performed by ClustVis. Rows are clustered using distance and average linkage. Changes in gene expression are displayed from white (higher expression) to black (lower expression)