Table 2 Identification of differentially (P < 0.05) phosphorylated 2-DE protein spots in DFD and control meat samples by MALDI-TOF and MALDI-TOF/TOF MS
From: The first evidence of global meat phosphoproteome changes in response to pre-slaughter stress
Spot no.a | Type of meat | Protein identityb | Abbrev. (isospot) | Accession | Mr (kDa) Obs./Expc | pI Obs./Expc | Scored | Sequence coveragee |
|---|---|---|---|---|---|---|---|---|
1 | DFD | Phosphoglucomutase-1 | PGM1 (1) | PGM1_BOVIN | 66.2/61.8 | 5.90/6.36 | 415 | 45 |
5 | DFD | Unidentified | Â | Â | 69.9/61.8 | 6.98/6.36 | Â | Â |
Control | Phosphoglucomutase-1 | PGM1 (2) | PGM1_BOVIN | 69.9/61.8 | 6.98/6.36 | 552 | 41 | |
6 | Control | Cytochrome b-c1 complex subunit 1, mitocondrial | UQCRC1 | QCR1_BOVIN | 53.9/53.4 | 5.60/5.94 | 364 | 47 |
7 | DFD | Beta-enolase | ENO3 (1) | ENOB_BOVIN | 51.9/47.4 | 6.33/7.60 | 269 | 39 |
8 | DFD | Beta-enolase | ENO3 (2) | ENOB_BOVIN | 51.8/47.4 | 6.45/7.60 | 253 | 34 |
9 | DFD | Beta-enolase | ENO3 (3) | ENOB_BOVIN | 51.7/47.4 | 6.63/7.60 | 269 | 48 |
10 | DFD | Actin, alpha skeletal muscle | ACTA1 (1) | ACTS_BOVIN | 42.2/42.4 | 4.95/5.23 | 474 | 62 |
11 | DFD | Actin, alpha 1, skeletal muscle | ACTA1 (2) | A4IFM8_BOVIN | 41.6/42.4 | 4.99/5.23 | 439 | 51 |
15 | DFD | Creatin kinase M-type | CKM (1) | KCRM_BOVIN | 45.0/43.2 | 6.34/6.63 | 266 | 39 |
16 | DFD | Creatin kinase M-type | CKM (2) | KCRM_BOVIN | 44.1/43.1 | 6.42/6.63 | 369 | 49 |
18 | Control | Actin, alpha, skeletal muscle | ACTA1 (3) | ACTS_BOVIN | 45.3/42.4 | 5.58/5.23 | 519 | 43 |
19 | Control | Actin, alpha, skeletal muscle | ACTA1 (4) | ACTS_BOVIN | 44.5/42.4 | 5.78/5.23 | 453 | 39 |
20 | Control | Actin, alpha, skeletal muscle | ACTA1 (5) | ACTS_BOVIN | 40.0/42.4 | 5.80/5.23 | 423 | 33 |
21 | Control | Actin, alpha, skeletal muscle | ACTA1 (6) | ACTS_BOVIN | 36.5/42.4 | 5.45/5.23 | 484 | 53 |
22 | DFD | Troponin T, fast skeletal muscle | TNNT3 (1) | TNNT3_BOVIN | 37.8/32.1 | 6.31/5.99 | 66 | 8 |
23 | DFD | Troponin T fast skeletal muscle type | TNNT3 (2) | TNNT3_BOVIN | 37.8/32.1 | 6.38/5.59 | 62 | 13 |
24 | DFD | Troponin T, fast skeletal muscle | TNNT3 (3) | TNNT3_BOVIN | 37.8/32.1 | 6.44/5.99 | 145 | 13 |
25 | DFD | Troponin T, fast skeletal muscle | TNNT3 (4) | TNNT3_BOVIN | 37.8/32.1 | 6.62/5.99 | 60 | 13 |
26 | DFD | F-actin-capping protein subunit alpha-2 | CAPZA2 | CAZA2_BOVIN | 35.4/33.1 | 5.48/5.57 | 74 | 16 |
27 | Control | Actin, alpha skeletal muscle | ACTA1 (7) | ACTS_BOVIN | 35.8/42.4 | 5.54/5.23 | 300 | 23 |
30 | Control | Troponin T, slow skeletal muscle | TNNT1 (1) | TNNT1_BOVIN | 33.8/31.3 | 6.53/5.71 | 109 | 20 |
30A | DFD | Troponin T, slow skeletal muscle | TNNT1 (2) | TNNT1_BOVIN | 35.5/31.3 | 5.63/5.71 | 71 | 14 |
33 | Control | Heat shock protein beta-1 | HSPB1 (1) | HSPB1_BOVIN | 25.8/22.4 | 5.05/5.98 | 230 | 36 |
34 | DFD | Heat shock protein beta-1 | HSPB1 (2) | E1BEL7_BOVIN | 26.2/22.6 | 5.09/5.77 | 159 | 20 |
37 | DFD | Heat shock protein beta-1 | HSPB1 (3) | E1BEL7_BOVIN | 25.9/22.6 | 5.34/5.77 | 163 | 27 |
44 | Control | Myosin, light chain 6B, alkali, smooth muscle and non-muscle | MYL6B (1) | Q148H2_BOVIN | 20.9/23.5 | 5.43/5.40 | 109 | 26 |
46 | Control | Myosin, light chain 6B, alkali, smooth muscle and non-muscle | MYL6B (2) | Q148H2_BOVIN | 21.1/23.5 | 5.69/5.40 | 329 | 58 |
50 | Control | Heat shock protein beta-6 | HSPB6 | HSPB6_BOVIN | 18.7/17.5 | 6.28/5.95 | 146 | 39 |
51 | Control | Myosin regulatory light chain 2, ventricular/cardiac muscle isoform | MYL2 (1) | MLRV_BOVIN | 18.1/18.9 | 4.86/4.86 | 399 | 65 |
52 | Control | Myosin regulatory light chain 2, ventricular/cardiac muscle isoform | MYL2 (2) | F1ME15_BOVIN | 18.2/18.9 | 4.90/4.86 | 380 | 76 |
53 | DFD | Myosin regulatory light chain 2, fast skeletal muscle isoform | MYLPF (1) | MLRS_BOVIN | 17.7/19.1 | 4.74/4.91 | 363 | 61 |
54 | DFD | Myosin regulatory light chain 2, fast skeletal muscle isoform | MYLPF (2) | MLRS_BOVIN | 17.6/19.1 | 4.77/4.91 | 88 | 23 |