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Table 2 Identification of differentially (P < 0.05) phosphorylated 2-DE protein spots in DFD and control meat samples by MALDI-TOF and MALDI-TOF/TOF MS

From: The first evidence of global meat phosphoproteome changes in response to pre-slaughter stress

Spot no.a Type of meat Protein identityb Abbrev. (isospot) Accession Mr (kDa) Obs./Expc pI
Obs./Expc
Scored Sequence coveragee
1 DFD Phosphoglucomutase-1 PGM1 (1) PGM1_BOVIN 66.2/61.8 5.90/6.36 415 45
5 DFD Unidentified    69.9/61.8 6.98/6.36   
Control Phosphoglucomutase-1 PGM1 (2) PGM1_BOVIN 69.9/61.8 6.98/6.36 552 41
6 Control Cytochrome b-c1 complex subunit 1, mitocondrial UQCRC1 QCR1_BOVIN 53.9/53.4 5.60/5.94 364 47
7 DFD Beta-enolase ENO3 (1) ENOB_BOVIN 51.9/47.4 6.33/7.60 269 39
8 DFD Beta-enolase ENO3 (2) ENOB_BOVIN 51.8/47.4 6.45/7.60 253 34
9 DFD Beta-enolase ENO3 (3) ENOB_BOVIN 51.7/47.4 6.63/7.60 269 48
10 DFD Actin, alpha skeletal muscle ACTA1 (1) ACTS_BOVIN 42.2/42.4 4.95/5.23 474 62
11 DFD Actin, alpha 1, skeletal muscle ACTA1 (2) A4IFM8_BOVIN 41.6/42.4 4.99/5.23 439 51
15 DFD Creatin kinase M-type CKM (1) KCRM_BOVIN 45.0/43.2 6.34/6.63 266 39
16 DFD Creatin kinase M-type CKM (2) KCRM_BOVIN 44.1/43.1 6.42/6.63 369 49
18 Control Actin, alpha, skeletal muscle ACTA1 (3) ACTS_BOVIN 45.3/42.4 5.58/5.23 519 43
19 Control Actin, alpha, skeletal muscle ACTA1 (4) ACTS_BOVIN 44.5/42.4 5.78/5.23 453 39
20 Control Actin, alpha, skeletal muscle ACTA1 (5) ACTS_BOVIN 40.0/42.4 5.80/5.23 423 33
21 Control Actin, alpha, skeletal muscle ACTA1 (6) ACTS_BOVIN 36.5/42.4 5.45/5.23 484 53
22 DFD Troponin T, fast skeletal muscle TNNT3 (1) TNNT3_BOVIN 37.8/32.1 6.31/5.99 66 8
23 DFD Troponin T fast skeletal muscle type TNNT3 (2) TNNT3_BOVIN 37.8/32.1 6.38/5.59 62 13
24 DFD Troponin T, fast skeletal muscle TNNT3 (3) TNNT3_BOVIN 37.8/32.1 6.44/5.99 145 13
25 DFD Troponin T, fast skeletal muscle TNNT3 (4) TNNT3_BOVIN 37.8/32.1 6.62/5.99 60 13
26 DFD F-actin-capping protein subunit alpha-2 CAPZA2 CAZA2_BOVIN 35.4/33.1 5.48/5.57 74 16
27 Control Actin, alpha skeletal muscle ACTA1 (7) ACTS_BOVIN 35.8/42.4 5.54/5.23 300 23
30 Control Troponin T, slow skeletal muscle TNNT1 (1) TNNT1_BOVIN 33.8/31.3 6.53/5.71 109 20
30A DFD Troponin T, slow skeletal muscle TNNT1 (2) TNNT1_BOVIN 35.5/31.3 5.63/5.71 71 14
33 Control Heat shock protein beta-1 HSPB1 (1) HSPB1_BOVIN 25.8/22.4 5.05/5.98 230 36
34 DFD Heat shock protein beta-1 HSPB1 (2) E1BEL7_BOVIN 26.2/22.6 5.09/5.77 159 20
37 DFD Heat shock protein beta-1 HSPB1 (3) E1BEL7_BOVIN 25.9/22.6 5.34/5.77 163 27
44 Control Myosin, light chain 6B, alkali, smooth muscle and non-muscle MYL6B (1) Q148H2_BOVIN 20.9/23.5 5.43/5.40 109 26
46 Control Myosin, light chain 6B, alkali, smooth muscle and non-muscle MYL6B (2) Q148H2_BOVIN 21.1/23.5 5.69/5.40 329 58
50 Control Heat shock protein beta-6 HSPB6 HSPB6_BOVIN 18.7/17.5 6.28/5.95 146 39
51 Control Myosin regulatory light chain 2, ventricular/cardiac muscle isoform MYL2 (1) MLRV_BOVIN 18.1/18.9 4.86/4.86 399 65
52 Control Myosin regulatory light chain 2, ventricular/cardiac muscle isoform MYL2 (2) F1ME15_BOVIN 18.2/18.9 4.90/4.86 380 76
53 DFD Myosin regulatory light chain 2, fast skeletal muscle isoform MYLPF (1) MLRS_BOVIN 17.7/19.1 4.74/4.91 363 61
54 DFD Myosin regulatory light chain 2, fast skeletal muscle isoform MYLPF (2) MLRS_BOVIN 17.6/19.1 4.77/4.91 88 23
  1. aGel position of assigned spots is shown in Fig. 1. bAll identified proteins were matched to Bos taurus proteins. cTheoretical (Th) isoelectric point (pI) and molecular mass (Mr) were obtained from UniProtKB/Swiss-Prot databases. Observed (Ob) pI and Mr were obtained from the spot position on the gel. dThe Mascot baseline statistically significant (P-value < 0.05) score was 56. ePercentage of coverage of the entire amino acid sequence by matched peptides