Fig. 3

Overexpression of Gh_A07G0747 (GhTOM1) effects on germination, root growth and fresh biomass accumulation in transgenic plants and wild type under osmotic stress: a Seed germination in 0.5 MS supplemented with 0, 100, 200 and 300 mM mannitol for 10 days. b Root length evaluation in 0.5 MS medium supplemented with 0, 100, 200 and 300 mM mannitol. c Quantitative comparisons of germination rate of the transgenic and WT on mannitol 0.5 MS with (0, 100, 200 and 300 mM) for 10 days. d Root elongation comparisons on mannitol 0.5 MS with (0, 100, 200 and 300 mM). e Fresh weight determination. Each experiment was repeated three times. Bar indicates standard error (SE). The seedlings were scored and photographed after 6 days post germination. Different letters indicate significant differences between wild-type and OE lines (ANOVA; p < 0.05). CK: normal conditions