Fig. 5

Overexpression of Gh_A07G0747 (GhTOM1) enhances cold stress tolerance: a Seed germination rate of transgenic lines and wild type on 0.5 MS at normal and low temperature (4 °C) for 10 days, in each set up, approximately 45 seeds were used to evaluate germination rate. b Quantitative determination of root lengths. c Quantitative comparisons of germination rate of the transgenic and wild type on 0.5MS at normal and low temperature (4 °C) for 10 days. d Root length evaluation on 0.5 MS at normal and low temperature (4 °C) for 10 days. e Quantitative determination of fresh weight biomass of wild-type and both overexpressed lines. The seedlings were scored and photographed after 6 days post germination. In each experiment three biological replications were done. Bar indicates standard error (SE). Different letters indicate significant differences between wild-type and OE lines (ANOVA; p < 0.05). CK: normal conditions