Fig. 5

qRT-PCR confirmation of differentially expressed miRNAs and their targets in adult gonads of Drosophila melanogaster. Dmel T: D. melanogaster treated with tetracycline (without Wolbachia); Dmel wMel: D. melanogaster infected with wMel Wolbachia. Red color indicates upregulation; Blue color indicates downregulation; Black color indicates not significantly different. A two-tailed Student’s t-test was performed to compare the significance between Wolbachia-infected and uninfected fly ovaries and testes of candidate miRNAs and their target genes. “/” represents the relative value; Bars indicate standard error, “*” and “**” indicate significant differences with p < 0.05 and p < 0.01, respectively. df = 4, n = 3