How do Wolbachia modify the Drosophila ovary? New evidences support the “titration-restitution” model for the mechanisms of Wolbachia-induced CI

Table 2 Differentially expressed genes selected for qRT-PCR validation in adult ovaries in the presence of Wolbachia

Relative expression level	Gene symbol	Log₂ Fold difference (Log₂ Dmel wMel/ Dmel T)	Biological functions
Up-regulated	uif	5.9542	Negative regulation of Notch signaling pathway
	RpL22-like	5.3421	Structural cosntituent of ribosome, protein metabolism
	dany	2.1552	Regulation of transcription from RNA polymerase II promoter involved in spermatogenesis
	otk2	1.0471	Imaginal disc-derived female genitalia morphogenesis
	CG32054	^aNo expression in Dmel T	Integral component of membrane
	CG10659	^aNo expression in Dmel T	Nitrogen compound metabolic process
Down-regulated	CG5111	−1.3823	Protein ubiquitination
	Twdlβ	−1.8867	Chitin-based cuticle development
	Def	−2.4344	Response to bacterium
	AttC	−2.5815	Response to bacterium
	CG18258	−3.2454	Lipid metabolic process
	nompC	−5.7343	Response to stimulus
	Cpr76Bd	−6.3724	Chitin-based cuticle development
	CG6435	^aNo expression in Dmel wMel	Lysozyme activity
	pgant8	^aNo expression in Dmel wMel	Oligosaccharide biosynthetic process

^aNo expression in Dmel T or Dmel wMel indicate that the gene can not be detected by RNA-seq

ISSN: 1471-2164