Fig. 6

Quantification of enriched genes and validation of the regulatory relationship between HSF1 and HSP70–2387 genes. a Quantification of the expression of six genes at different sampling times under 35 °C heat stress treatment (n = 5). The X axis represents the sampling time from the start of the experiment (ck represents 0 h from the start, and sampling times were 0, 1/4, 1/2, 1. 2, 6, 12, and 24 h). The Y axis on the left represents fold change of the genes presented in the histogram while the right axis represents fold change of the genes presented in the line chart. The different colors represent the fold changes of each gene. b FPKM values and the fold change of enriched genes. Control represents group 1, HS represents group 2, and RNAi&HS represents group 4. The X axis represents different genes. The Y axis on the left represents FPKM of HS (heat shock) and control groups, while the right axis represents HS/control (FPKM of heat shock divided by FPKM of control) and HS/RNAi&HS (FPKM of heat shock divided by FPKM of RNAi&HS). c Sequencing of the HSP70–2387 gene and the binding site of HSF1 predicted by AnimalTFDB 3.0. The red, blue, and black frames represent the predicted heat shock elements. d Relative luciferase activity of the HSF1 to the HSP70–2387 genes. The control had no HSF1 plasmid added to the system, and HSF1a and HSF1d represent the plasmids that can produce proteins containing the HSF1a and HSF1d isoforms of HSF1, respectively. The error bar denotes the standard error of the mean; **, p < 0.05; *** p < 0.001