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Table 4 Additional information about the identification of the clusters done with BLASTp searches against NCBI’s non-redundant database

From: Transcriptome annotation and characterization of novel toxins in six scorpion species

Cluster name Best BLASTp hit Signal peptide C pattern Conserved residues New cluster/singlet label
Cluster 1 Lamda-potassium channel toxin (ADT64271.1) Some Yesa High New toxins in the lamda-potassium channel toxin-family
Cluster 2 Hypothetical secreted protein (ADY39531.1) High Yesa High Novel putative toxin-family 1
Cluster 3 U6-buthitoxin-Hj1a (ADY39519.1) High Yesa High New toxins in the buthitoxin family
Cluster 4 Orphan peptide AbOp-11 (AIX87714.1) High N.A. High Novel putative secreted proteins
Cluster 5a Hypothetical secreted protein (ADY39514.1) High Yesa High Novel putative toxin-family 2
Cluster 6 venom peptide HtC4Tx1(AOF40173.1) Low Yesa Low Novel putative toxin-family 3
Cluster 7 hypothetical protein (WP_063562212.1) Low Noa Low Novel putative toxin-family 4
Cluster 8 Orphan peptide AbOp-18 (AIX87708.1) High N.A. High New toxins in the neuropeptide toxin-families
Cluster 9 Venom toxin meuTx23 (AMX81480.1) Low N.A. High New toxins related to meuTx23
Cluster 10 Hypothetical secreted protein (ADY39511.1) High N.A. Low Novel putative secreted proteins
Cluster 11 RNA-binding protein, putative (SCO66159.1) Low N.A. Some Novel putative secreted proteins
Cluster 12 Uncharacterized protein (XP_023221782.1) High Yesa High Novel putative toxin-family 5
Cluster 13 Potassium channel toxin alpha-KTx 4.5 (Q5G8B6.1) Low Yesa High New toxins in the potassium channel toxin alpha-KTx 4.5 toxin family
Cluster 14 Hypothetical protein (AEX09189.1) High N.A. Some Novel putative secreted proteins
Cluster 15 Hypothetical protein (GAU10035.1) Low Yesa Some Novel putative short toxin family 6
Singlet 1 No Hit N.A N.A N.A Novel putative secreted protein
Singlet 2 No Hit N.A. N.A. N.A. Novel putative secreted protein
Singlet 3 Hypothetical protein (AEX09189.1) High N.A. Some Novel putative secreted protein
Singlet 4 Orphan peptide AbOp-11 (AIX87714.1) High N.A. Low Novel putative secreted protein
Singlet 5 Potassium channel toxin kappa-KTx (P0DJ41.1) Some Yesa Low New potassium channel toxin
Singlet 6 Venom peptide Htgkr2 (AOF40260.1) Some N.A. Low Novel putative secreted protein
Singlet 7 No Hit N.A N.Aa N.A Novel putative toxin 1
Singlet 8 SH3 domain and tetratricopeptide repeat-containing protein (XP_004574858.2) Low N.A. Low Novel putative secreted protein
Singlet 9 Putative antimicrobial peptide (AEX09192.1) High N.A. High Novel putative AMP
  1. aIndicates clusters or singlets with a conserved C pattern