Table 4 Additional information about the identification of the clusters done with BLASTp searches against NCBI’s non-redundant database
From: Transcriptome annotation and characterization of novel toxins in six scorpion species
Cluster name | Best BLASTp hit | Signal peptide | C pattern | Conserved residues | New cluster/singlet label |
|---|---|---|---|---|---|
Cluster 1 | Lamda-potassium channel toxin (ADT64271.1) | Some | Yesa | High | New toxins in the lamda-potassium channel toxin-family |
Cluster 2 | Hypothetical secreted protein (ADY39531.1) | High | Yesa | High | Novel putative toxin-family 1 |
Cluster 3 | U6-buthitoxin-Hj1a (ADY39519.1) | High | Yesa | High | New toxins in the buthitoxin family |
Cluster 4 | Orphan peptide AbOp-11 (AIX87714.1) | High | N.A. | High | Novel putative secreted proteins |
Cluster 5a | Hypothetical secreted protein (ADY39514.1) | High | Yesa | High | Novel putative toxin-family 2 |
Cluster 6 | venom peptide HtC4Tx1(AOF40173.1) | Low | Yesa | Low | Novel putative toxin-family 3 |
Cluster 7 | hypothetical protein (WP_063562212.1) | Low | Noa | Low | Novel putative toxin-family 4 |
Cluster 8 | Orphan peptide AbOp-18 (AIX87708.1) | High | N.A. | High | New toxins in the neuropeptide toxin-families |
Cluster 9 | Venom toxin meuTx23 (AMX81480.1) | Low | N.A. | High | New toxins related to meuTx23 |
Cluster 10 | Hypothetical secreted protein (ADY39511.1) | High | N.A. | Low | Novel putative secreted proteins |
Cluster 11 | RNA-binding protein, putative (SCO66159.1) | Low | N.A. | Some | Novel putative secreted proteins |
Cluster 12 | Uncharacterized protein (XP_023221782.1) | High | Yesa | High | Novel putative toxin-family 5 |
Cluster 13 | Potassium channel toxin alpha-KTx 4.5 (Q5G8B6.1) | Low | Yesa | High | New toxins in the potassium channel toxin alpha-KTx 4.5 toxin family |
Cluster 14 | Hypothetical protein (AEX09189.1) | High | N.A. | Some | Novel putative secreted proteins |
Cluster 15 | Hypothetical protein (GAU10035.1) | Low | Yesa | Some | Novel putative short toxin family 6 |
Singlet 1 | No Hit | N.A | N.A | N.A | Novel putative secreted protein |
Singlet 2 | No Hit | N.A. | N.A. | N.A. | Novel putative secreted protein |
Singlet 3 | Hypothetical protein (AEX09189.1) | High | N.A. | Some | Novel putative secreted protein |
Singlet 4 | Orphan peptide AbOp-11 (AIX87714.1) | High | N.A. | Low | Novel putative secreted protein |
Singlet 5 | Potassium channel toxin kappa-KTx (P0DJ41.1) | Some | Yesa | Low | New potassium channel toxin |
Singlet 6 | Venom peptide Htgkr2 (AOF40260.1) | Some | N.A. | Low | Novel putative secreted protein |
Singlet 7 | No Hit | N.A | N.Aa | N.A | Novel putative toxin 1 |
Singlet 8 | SH3 domain and tetratricopeptide repeat-containing protein (XP_004574858.2) | Low | N.A. | Low | Novel putative secreted protein |
Singlet 9 | Putative antimicrobial peptide (AEX09192.1) | High | N.A. | High | Novel putative AMP |