Fig. 3

Phylogenetic tree of reference and novel virus assemblies from the Mononegavirales order. a Maximum-likelihood phylogeny based on RNA-dependent RNA polymerase (RdRP) predicted peptide sequences of viruses from Mononegavirales order. Novel virus assemblies characterized from Cambodia and Senegal Anopheles samples (red name labels) are placed with reference viruses (black name labels), predominantly within the Dimarhabdovirus clade and as close relative of the Nyamivirus clade. Node robustness is indicated by bootstrap values (number of replicates supporting the node), indicated by color of the dot at the branch base, see key. Protein lengths and functional status of RdRP peptide sequences from novel viruses in the current study are included to distinguish between complete and partial and/or non-functional pseudogenes (indicated by label “pseudo”, functional status indicated in Additional file 2: Table S2 and Additional file 3: Table S3). Average protein size of reference virus RdRP genes is 2098 amino acids. b Genome comparison of novel and reference Xincheng Mosquito Viruses, which are too diverged to align at the nucleic acid sequence level. Grey blocks represent peptide sequence homology regions between compared sequences. The nucleotide sequences of the entire viral contigs, and not only of the RdRP gene as in (a), were translated and used to search the translated nucleotide database with TBLASTX. The viruses display recognizable relatedness over their genomes, despite geographic distance and nucleotide sequence divergence. Color intensity indicates identity levels from TBLASTX results (values indicated in key)