Fig. 2

Mean methylation levels and differences across all DMRs. Histograms of average methylation levels within all 709 DMRs, and how they were different across social status. Means and medians of each distribution are reported in the plot and represented by vertical solid and dashed red lines, respectively. All values for individual DMRs are reported in Additional file 2: Table S1. a-b: Averages of smoothed methylation levels across all cytosines within each DMR for ND (a) and D (b) fish. c: Differences between average smoothed methylation levels for ND - D fish, such that negative values reflect higher methylation in D fish. DMRs where the absolute value of this difference was less than 0.1 were filtered out during quality control (Methods). d: Log2 ratio (fold-difference) of average smoothed methylation values for D / ND fish, such that positive values reflect higher methylation in D fish