Fig. 2

Clustering analysis of the cerebellar development time-points. a) Bioanalyzer graph shows the RNA integrity of our cerebellar RNA samples. The two peaks in the center represent 18S and 28S rRNA respectively and a RIN score (max of 10) is measured by aggregating the electrophoretic trace of the RNA sample. b) Hierarchical clustering. Hierarchical clustering of cerebellar development on time points (mean expression of three biological replicates) show three major groupings. The very early embryonic time points (E11 and E12) are the most distant from other late embryonic (E13-E18, P0) and postnatal (P3-P9) time points. c) Principal component analysis (PCA). PCA on cerebellar development shows a clear temporal trajectory from early embryonic E11 to postnatal time point, P9