Fig. 3

The validation of the reclassifications by the signature for fresh frozen samples with survival data. a Kaplan-Meier curves of overall survival (OS) respectively for the pADC reclassified as SCC and the signature-confirmed pADC groups. b Kaplan-Meier curves of OS respectively for the pSCC reclassified as ADC and the signature-confirmed pSCC groups. c, d Kaplan-Meier curves of OS respectively for the SCC and ADC groups reclassified by the signature (c) and original pathological assessment (d). e The violin plot of proliferation scores in the reclassified and signature-confirmed samples, respectively, in the GSE50081 dataset with the higher reclassification rate in the fresh frozen samples. Wilcoxon rank sum test was used to test the difference of proliferation scores between two groups. f The violin plot of mRNA expressions of the seven subtype-specific marker genes in the GSE50081 dataset. The subtype-specific marker genes include ADC marker genes (NAPSA, TTF1), SCC marker genes (KRT5, TP63) and neuroendocrine marker genes (CD56, SYP, CHGA). The RankProd (RP) algorithm was used to test the difference of the subtype-specific marker genes between reclassified samples and signature-confirmed samples