Fig. 1
From: Continuous salt stress-induced long non-coding RNAs and DNA methylation patterns in soybean roots
Flowchart of lncRNA identification in soybean roots. Transcripts were subject to a series of steps for excluding known protein-coding RNAs, house-keeping RNAs, and miRNA precursors. Besides, CPC program and Swiss-Prot database were used for accessing their coding potentials and further filtration. Low expressed (FPKM < 2) and short transcripts (< 200 nt) were also discarded. At last, transcripts located on the opposite strands of known genes were classified as lncNATs, while novel intergenic transcripts 200 nt away from coding regions were regarded as lincRNAs