Fig. 3

a. Dose-effect curves in zygotes injected with various concentrations of TRIM21 protein or mCherry-Trim21 mRNA (mg/mL) in a volume of ≈ 100 picoliters. N = 43 zygotes were inspected for each mRNA concentration, N = 22 zygotes for each protein concentration. b Q-PCR for Trim21 sequence conducted at 24-h intervals after microinjection of mCherry-Trim21 mRNA in the zygote. N = 5 embryo equivalents from a lysate of 20 embryos per stage. Height of bars indicates the excess amount of microinjected Trim21 mRNA over the endogenous Trim21 mRNA. c The fluorescent protein product of mCherry-Trim21 mRNA is visible already 3 h after microinjection and accumulates in the blastocysts. d Stability of microinjected antibody in the absence of mCherry-Trim21 mRNA, demonstrated via immunofluorescence against primary antibody in zygotes microinjected with anti-GFP antibody. Left, representative picture of immunofluorescence against microinjected anti-GFP; right, measured fluorescence intensity (n = 7 embryos per time point). Size bar, 50 μm. AU, arbitrary units of fluorescence intensity