Fig. 6

a. Morphologies of blastocysts and outgrowths with and without TEAD4 depletion. b. Developmental rates during preimplantation and outgrowth formation. Zygotes injected with mCherry-Trim21 mRNA, n = 212; zygotes injected with mCherry-Trim21 mRNA and anti-GFP antibody, n = 245; zygotes injected with mCherry-Trim21 mRNA and anti-TEAD4 antibody, n = 501. Blastocysts tested for outgrowth formation: n = 12 after mCherry-Trim21 mRNA, n = 8 after mCherry-Trim21 mRNA and anti-GFP antibody, n = 12 after mCherry-Trim21 mRNA and anti-TEAD4 antibody. c. Developmental rates after blastocyst transfer to uterus and representative pictures of uteri. Postimplantation development of TEAD4-depleted embryos was lower than that embryos injected with mCherry-Trim21 mRNA or with mCherry-Trim21 mRNA + anti-GFP antibody. Day E3.5 blastocysts containing mCherry-Trim21 mRNA, n = 30 in three recipients; day E3.5 blastocysts containing mCherry-Trim21 mRNA and anti-TEAD4 antibody, n = 30 in three recipients; day E3.5 blastocysts containing mCherry-Trim21 mRNA and anti-GFP antibody, n = 48 in five recipients. Implantation rate: (fetuses + empty decidua + miscarriages) / transferred embryos. Fetal rate: fetuses / transferred embryos (see Methods). d. Raw Affymetrix signal intensities of selected mRNAs in blastocysts, in logarithmic scale. Two pools of 10 blastocysts each were analyzed for each group (mCherry-Trim21 mRNA, anti-GFP, anti-TEAD4). Size bar, 50 μm. OGDB, Oregon Green dextran beads. Error bars = standard deviations. Statistical significance tested with Student’s t test. n.s., not significant. AU, arbitrary units of Affymetrix hybridization intensity