Fig. 5

Stable vegetative growth of the Magnaporthe oryzae wildtype strain (WT 70–15), mutants with inactivated components of the HOG signaling cascade and the “adapted” strains upon fludioxonil treatment. The fungal colonies were grown on minimal medium (MM, upper row) and on MM including 10 μg/ml fludioxonil (lower row) for 7 d at 26 °C. A, B and C are representative for each plate. A = lof mutant, B = “adapted” strain taken directly from CM including 1 M KCl (“adaptation” conditions) and C = “adapted” strain after six weeks of preincubation on CM (to have long-term unstressed conditions before this assay to show the memory effect). In the case of the wildtype strain (left), the three cultures on each test plate used in the assays were from the M. oryzae wildtype strain 70–15 cultivated in an equal manner