Fig. 1

Quantity and quality of DNA by variable methods. a Total DNA by Qubit® and b 260/280 and c 260/230 by NanoDrop 2000 UV-Vis Spectrophotometer. All methods performed in triplicate with error bars (standard deviation). All methods produced sufficient total DNA except Method 6 (a). For (b) and (c), horizontal bars representing significance values for one-way ANOVA with Tukey’s post-hoc multiple comparisons test against Method 5. Significance in p-values as follows: 0.033 (*), 0.002 (**), < 0.001 (***). DNA molecular weight demonstrated by (d) gel electrophoresis of genomic DNA on a 0.6% ethidium bromide gel of extractions by Methods 1–7 in triplicate (a, b, c) and (e) Bioanalyzer of Method 1 and Method 5 in triplicate