| Â | Total reads | Total bases | Mean read length (bp) | Median read length (bp) | Longest read | Phred score | %Error Probabilitya |
|---|
Method 1 | 1,455,977 | 1,634,706,997 | 1122.8 | 751.0 | 34,548 | 13 | 5.011% |
Method 5 | 1,500,966 | 2,778,573,523 | 1851.2* | 1196.0* | 64,327 | 14 | 3.981% |
- Raw values are listed for each MinION ONT runs using DNA extracted by Method 1 (after additional clean-up) and by Method 5. For statistical analyses comparing Method 1 (n = 3) versus Method 5 (n = 3), paired-t test comparisons (total reads, total bases, mean read length, median read length, longest read) were completed on basecalled, demultiplexed, and trimmed reads. Mean read length and median read length were significantly longer from Method 5, p = 0.0168 and p = 0.0101, respectively. Notably, Phred score was higher and error probability lower in the Method 5 run, which may reflect higher quality substrates
- * Indicates p-value of p <  0.05
- a Error probability percentage is a function of Mean Phred score, where probability P% = 100*10^(−Phred/10)
