Fig. 5
Phenotypes, molecular identification and SbER2–1 expression level in transgenic plants. a Phenotype identification of T3 Arabidopsis plant transformed with SbER2–1. NT, non-transgenic (wild type); OE-SbER2–1, transgenic plants; CG, control group with normal irrigation; EG, experimental group treated with PEG. b Plant aboveground phenotypes of transgenic maize lines VE2–1 and VE2–2, as well as NT ZPM9 (CK), under WW (left), MS (middle), and SS (right) conditions. c PCR detection of transgenic lines by agarose gel electrophoresis. Transgenic plants (1–5 lane) and positive control (+) show amplified 506-bp fragment; negative control (−) and blank control (b) had no amplified fragment. d SbER2–1 expression level of transgenic maize plants under WW, MS, and SS conditions. e, f Drought-tolerance index (DTI) of shoot biomass of VE2–1, VE2–2, and ZPM9 maize under MS (e) and SS (f) conditions, respectively