Fig. 4

Detailed characterization of Similar to 5′-3′ Exoribonuclease (XRN4) gene (Potri.005G048900) associated with leaf δ¹⁵N. a Manhattan plot for leaf δ¹⁵N highlighting (red circle) the window containing significant SNPs for the gene. The horizontal blue line indicates a referential -log₁₀ (p-value) of 2 (equivalent to p-value = 0.01). b LD heat map for the analyzed SNPs located at gene. Red bars at the top correspond to SNPs identified as significantly associated with δ¹⁵N by single-marker association tests. c Detailed view for the light blue triangle depicted in b). Numbers 1, 2, 3 and 4 are the markers S05_3547832, S05_3547864, S05_3547904 and S05_3548573, respectively. Boxplots shows the effects of genotypes on leaf δ¹⁵N. Different letters indicate significant differences among adjusted means (Tukey’s HSD test; α = 0.001)