Table 5 Primers designed for PCR amplification
Primer name | Primer sequence | Primer size (bp) | Tm (°C) | Amplicon expected size (bp) |
|---|---|---|---|---|
isotig42940_1F | GAGGCTGATCCCAAGAGATAAT | 23 | 60.3 | 102 |
isotig42940_1R | AAATTGTACTGGCCAAAGTGT | 22 | 55.5 | |
isotig18002_F | AACCAGTGCTAAAGTCCTTGAT | 22 | 58.4 | 164 |
isotig18002_R | CTGCTAGCAATGGTGGTCAT | 20 | 58.4 | |
isotig37981_F | AGCAGCAAGCAGAGCAA | 17 | 52.4 | 210 |
isotig37981_R | CAGATGTGGCCAGGATAGATG | 21 | 61.2 | |
isotig40670_F | GGATTGTGCTCTCTCTCTTCTG | 22 | 61.2 | 212 |
isotig40670_R | GTGTTCATCTGCTCGCTCT | 19 | 57.5 | |
isotig39554_1F | TCTGGCTGTCGAAGGATCTA | 21 | 58.4 | 127 |
isotig39554_1R | GGATGTTCTCCATGGTTGGT | 21 | 58.4 | |
ecu-miR156a | TTGACAGAAGATAGAGAGCAC | 22 | 57.4 | – |
ecu-miR156b | GCTCTCTATGCTTCTGTCATC | 22 | 59.4 | – |
ecu-miR2275a | TTGTTTTTCTCCAATATCTC | 21 | 50.2 | – |
ecu-mir2275b | TTCAGTTTCCTCTAATATCTC | 22 | 53.5 | – |
ecu-miR396 | TCCACAGGCTTTCTTGAACT | 21 | 56.4 | – |
ecu-miR8175 | TCCCCGGCAACGGCGCCA | 19 | 65.3 | – |
ecu-miR827 | TTAGATGACCATCAGCAAACA | 22 | 55.5 | – |
ecu-miR894 | GTTTCACGTCGGGTTCACCA | 21 | 60.5 | – |
micro_isotig42940 | ACAACTAGCAAGATGGCCCGCGC | 24 | 68.2 | – |
micro_isotig46613_F | ggcggcgTATATTTTGAAACAG | 22 | 60.1 | – |
micro_isotig46613_s_loop | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACctccct | 50 | – | – |
micro_isotig28724_F | cggcgGAACTCGTAGAGCTT | 20 | 62.5 | – |
micro_isotig28724_s_loop | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACcgcggc | 50 | – | – |
URP | CCAGTGCAGGGTCCGAGGT | 19 | 63.6 | – |