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Table 1 Comparison of five different DNA extraction methods for microbiota analysis on the basis of cost, concentration, and the time duration for sample processing

From: Analysis of the fecal microbiota of fast- and slow-growing rainbow trout (Oncorhynchus mykiss)

Extraction Kit Manufacturer Principle Bead Beating Concentration (ng/μl) A260/230 Cost per sample Time duration Hazardous waste
Power Soil MoBio Manual Yes 6.49 ± 9.09 1.78 ± 0.18 $6.48 6 h Moderate
Maxwell Promega Automated Yes 28.76 ± 12.44 1.72 ± 0.17 $7.40 1.5 h Least
Phenol:Chloroform Sigma Manual No 257.1 ± 285.0 1.73 ± 0.08 $4.50 2 days High
Qiagen_Stool Qiagen Manual No 25.1 ± 10.07 1.92 ± 0.16 $5.60 5 h Less
Qiagen_Blood/Tissue Qiagen Manual No 35.2 ± 2.7 1.72 ± 0.01 $4.20 5 h Less