Table 1 Comparison of five different DNA extraction methods for microbiota analysis on the basis of cost, concentration, and the time duration for sample processing
From: Analysis of the fecal microbiota of fast- and slow-growing rainbow trout (Oncorhynchus mykiss)
Extraction Kit | Manufacturer | Principle | Bead Beating | Concentration (ng/μl) | A260/230 | Cost per sample | Time duration | Hazardous waste |
|---|---|---|---|---|---|---|---|---|
Power Soil | MoBio | Manual | Yes | 6.49 ± 9.09 | 1.78 ± 0.18 | $6.48 | 6 h | Moderate |
Maxwell | Promega | Automated | Yes | 28.76 ± 12.44 | 1.72 ± 0.17 | $7.40 | 1.5 h | Least |
Phenol:Chloroform | Sigma | Manual | No | 257.1 ± 285.0 | 1.73 ± 0.08 | $4.50 | 2 days | High |
Qiagen_Stool | Qiagen | Manual | No | 25.1 ± 10.07 | 1.92 ± 0.16 | $5.60 | 5 h | Less |
Qiagen_Blood/Tissue | Qiagen | Manual | No | 35.2 ± 2.7 | 1.72 ± 0.01 | $4.20 | 5 h | Less |