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Fig. 5 | BMC Genomics

Fig. 5

From: Multiple transcription factors co-regulate the Mycobacterium tuberculosis adaptation response to vitamin C

Fig. 5

DevR and Rv0348 co-regulate DevR-dependent genes. a Rv3134c promoter region. Rv3134c-devR-devS operon genes are depicted by black arrows and the promoter region consists of primary (P) and secondary (S) Dev boxes where DevR binds to the promoter. The DNA probe for EMSA depicted by the black box was generated from the promoter region by PCR amplification. b EMSA of the Rv3134c promoter fragment in the presence of Rv0348 and phosphorylated DevR (DevR~P). Formation of the complex of DevR alone with DNA and a higher molecular weight complex of Rv0348, DevR and DNA are depicted on the left. c EMSA of the Rv3134c promoter fragment in the presence of Rv0348 and unphosphorylated DevR (DevR-unP). d. Interaction of DevR-unP (3.8 to 19 pmol) and Rv0348 (15 pmol) proteins demonstrated by ELISA. BSA was used as a negative control in the assay

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BMC Genomics

ISSN: 1471-2164

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