Fig. 7

Expression Levels of Four Candidate miRNAs in different stages. a Expression of four candidate miRNAs from RNA-seq calculated by the TPM method. The expression is shown as log₂ (TPM). b Expression of four candidate miRNAs from qPCR. The qPCR results are presented at each stage as the mean ± SD. The significant differences (P < 0.05) among the PCR results in different groups were indicated using different letters on each bar. EI (black bars), EII (red bars) and EIII (green bars) indicate pre-elongation stage, elongation stage and rapid elongation stage, respectively