Fig. 4

Effects of filtering multi-mapped reads in the calculation of gene expression of the flavonoid 3′ and 3′,5′ hydroxylase gene families in different somatic color variants. The cv. ‘Moscatel Galego’ experiment was coupled to RNA-Seq data generated in cv. ‘Red Globe’ (RG) and its less-pigmented color variant cv. ‘Chimenti Globe’ (CG; Santibañez et al., 2019). a PCA plot showing the effect of filtering, produced from logFPKM+ 1 values of both hydroxylase gene families. Clustering of samples according to genotype, developmental stage and read mapping procedure is shown. b Heatmap of logFPKM+ 1 transformed data comparing multi-mapped read filtering (f) and non-filtering (m). Samples (columns) are clustered using correlation distance and average linkage