Transcriptome profiling of resistance response to Meloidogyne chitwoodi introgressed from wild species Solanum bulbocastanum into cultivated potato

Table 3 Summary of top ten gene primers used in qPCR validation of the transcriptomic data generated in the present study

Gene code	Gene Name	Forward Primer	Reverse Primer	Tm (°C)
Gene26	Basic PR-1 protein	CTG TAG GAT GCA ACA CTC TGG TGG C	CCA AGA CGT ACT GAG TTA CGC CAG	58
Gene17	Proteinase inhibitor type-2	CAA GGC ATG TAC CCT GGA ATG TGA C	GGC TCT CCA GTA CAA ATT AAA GAT CCA TC	57
Gene 19	Invertase inhibitor	GAT GGT ATG GAT GAT GTT GTT GTT GAA GC	GCA ACT TTT GAT AGT TCA ATT ATT TCC CTA CTC	57
Gene25	Glutathione S-transferase	CTG ATC CTT ATG AGA GAT CAC AAG CC	GCT TCC TCC AGT AAC TTG AGT GG	57
Gene40	Replication factor A protein	GCA CAA ATG TCA TCA GCA GCT TC	GCA TCC TGA GCA TTC AAG CAC	57
Gene89	Lectin protein	GAA GTG GCT GAG CTT GTT AGA ACT TG	GCC TTT TCA AGT CCA TGT GAA TCC TC	58
Gene100	BEL5 protein	GTG GAT CAA AGG TAT AGA CAA TAC CAT CAC C	GAA ATT GTG TGC AAA GCA AGT TGT GTG	58
Gene53	Gene of unknown function	CAC CAC GTA GAT CCC TCT ACC TTA G	CAT GAT CCA CGA TCA GGT GAC G	58
Gene32	Mitogen-activated protein kinase 4	CGG AGA ATC TAC GAG CAG TGG CG	CAC AGA TTC CCT CCA AAT GAG CTC C	57
Gene43	TOM20	CTT GGC GAG GTG GGG ACG	CCC AAA CAC CAA AGC ACA TCA TGC	60

ISSN: 1471-2164