Table 4 List of oligonucleotides
Name | Sequence (5′ → 3′) | Purpose |
|---|---|---|
Cas1_fw | GCGCGCGGCTGGCGCGA | Detection of cas1 gene |
Cas1_rev | CGGCGATTGCGTCCGCC | |
Leader_fw | TCACGGGGTCCGCATGAC | PCR amplification of CRISPR array |
Terminator_rev | CTCGTCAGCGTCCGGCTG | |
Spacer#19_fw | CGAGCGCATCGATGACGG | PCR amplification of internal region of the CRISPR array |
Spacer#21_fw | TCGGGTTTCGGGATGTGC | |
Spacer#02_rev | CCGGGACGGCGACGAAC | |
Spacer#18_rev | CGTCGCTGTCGGTGGCAC | |
IS-1_rev | ACCAGCGCCAGCAGCGG | PCR amplification of internal region of the CRISPR array next to an IS element |
IS-2_fw | GCCGACCTGATGATGCA |