Fig. 4

The growth arrest recovery translatome of O. dioica. Animals were cultured under dense conditions before being released by dilution in the presence of food. a Median translational efficiency (RPF/mRNA = ribosome protected fragment density/mRNA density) of mRNAs from 2 replicates for growth arrested and released animals with transcripts identified as having significantly up- or down-regulated translation highlighted. b Degree of change in translational efficiency upon release from stasis (y-axis) against log fold change in mRNA abundance (x-axis) with known mTOR-independent (histone mRNAs) and mTOR target (ribosomal protein mRNAs) gene categories highlighted. The majority of mTOR targets are not up-regulated upon release from stasis. c-g EdU incorporation (DNA replication: green) was restored in the germline (top in each image, as indicated by arrows) 12 h after animals were released from the crowded conditions of growth arrest independent of food supply confirming that a change in density rather than increased food availability is the primary trigger for exiting a growth-arrested state in O. dioica (DNA was counterstained with blue To-Pro-3 iodide). c normal day 3; d growth arrest; e release without food; f release with food; g number of animals showing extensive DNA synthesis under all four conditions (C = normal day 3 control; S = stasis; R = release without food; RF = release with food)