Fig. 1

Effects of fasting and refeeding on zebrafish body weight and hepatocyte morphology. a Growth curve of zebrafish during fasting and refeeding. Arrows represent the start of fasting and refeeding, respectively; asterisks denote significant differences between fasting and the control group at the same stage (P < 0.05), n = 5–6. b Representative gross liver tissues from zebrafish fed ad libitum (ctrl), fasted for 3 weeks (fasted) and re-fed 3 days after a 3-week fast (refed). Scale bar, 2 mm. c H&E staining of liver samples from fed ad libitum (ctrl) and re-fed for 3 days following a 3-week starvation (refed). d Western blot analysis of PCNA expression in liver of zebrafish fed ad libitum (ctrl) and re-fed 3 days after a 3-week fast (refed), n = 4. e Triglyceride (TG) content in zebrafish liver when refed for 3 days (R3d) and 10 days (R10d) after a 3-weeks fasting, n = 4. f Glycogen content in zebrafish liver when refed for 3 days (R3d) and 10 days (R10d) after a 3-weeks fasting, n = 4–6. Error bars were ± SEM. For d, e and f, asterisks denote significant differences between refed group and the control group (P < 0.05)