Fig. 13

Validation of Pseudomonas aeruginosa (PA) inoculum by BAL culture post-inoculation. PA was inoculated into the trachea at a dose of 1 × 10⁶ CFU/mouse and following administration BAL was collected at 6 or 24 h, post-inoculation and plated out on sheep blood agar plates. Bacterial colony count was done after 24 h. We noted that there was no significant difference following administration of PA by intratracheal route as the BAL at 6 or 24 h showed comparable number of bacterial colony count suggesting no difference in the bacterial load inoculated. WT mice showed a mean colony count of 46.3 × 10 ⁴/ml and the Sphk2 KO 44.55 × 10 ⁴/ml at 6 h post-inoculation and at 24 h WT had a mean colony count of 3.05 × 10 ⁴/ml and Sphk2 KO, 3.16 × 10 ⁴/ml