Fig. 3

Receiver operating characteristic (ROC) curves for two sets of variables used to predict SNP genotyping success in Douglas-fir. a Shows the predictive ability of variables used to design the Axiom array (Table 3). Some of these variables were calculated using an earlier version of the Douglas-fir reference genome (v0.5) [16]. b Shows the predictive ability of alternative design variables. We replaced some of the original design variables with new variables calculated using v1.0 of the Douglas-fir reference genome [16], resulting in the final selected variables described in Table 3. ROC curves are used to evaluate binary predictive models (e.g., predictions of SNP success versus failure). Successful SNPs were those that had a call rate > 60% and were polymorphic