Fig. 1

PFGE pattern of chromosomal and plasmid DNA of B. miyamotoi isolate Izh-4 in three independent repetitions. N1-N9 indicate PFGE fragments which were subjected to gel extraction and sequencing via the Illumina platform. The name of plasmids with corresponding length is given on the right site of the gel. It was based on the comparison of assembled contigs from each of the PFGE fragments with the final assembly. Of note, the lp6 plasmid did not separate in PFGE, no distinct band at that size was visible. This may have been due to insufficient PFGE conditions, as lp6 sequences were identified in the fragment of 13 kb together with plasmid lp13 by direct sequencing