Table 1 Sequence coverage at variable nucleotide positions between eIF4E sequences in the ATL07 and ATLWT plants. Sequence coverages of the eIF4E pvr12 mutations (T209A, G245T, A334G) are represented in bold. Raw depth corresponds to the total nucleotide count at each position. A1 represents the most frequent nucleotide observed at that position and A2 the second most abundant. The relative abundance of each nucleotide is shown in parentheses
Position | ATL07 | ATLWT | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Raw Depth | A1 | Depth (%) | A2 | Depth (%) | Raw Depth | A1 | Depth (%) | A2 | Depth (%) | |
68 | 978 | C | 976 (99.8) | G | 1 (0.1) | 130 | C | 107 (82.3) | G | 22 (16.9) |
78 | 987 | A | 973 (98.6) | G | 13 (1.3) | 168 | A | 128 (76.2) | G | 40 (23.8) |
131 | 2233 | C | 2207 (98.8) | T | 20 (0.9) | 602 | C | 454 (75.4) | T | 146 (24.3) |
144 | 1530 | A | 1503 (98.2) | G | 27 (1.8) | 521 | G | 270 (51.8) | A | 251 (48.2) |
165 | 182 | A | 163 (89.6) | G | 18 (9.9) | 301 | G | 300 (99.7) | – |  |
209 | 1538 | A | 1464 (95.2) | T | 71 (4.6) | 611 | T | 611 (100) | – |  |
245 | 2924 | G | 2847 (97.4) | T | 72 (2.5) | 590 | T | 587 (99.5) | – |  |
279 | 3009 | T | 2946 (97.9) | G | 60 (2.0) | 646 | G | 394 (61.0) | T | 250 (38.7) |
334 | 2240 | A | 2144 (95.7) | G | 90 (4.0) | 702 | G | 695 (99.0) | – |  |
413 | 7614 | A | 7359 (96.6) | G | 245 (3.2) | 1487 | G | 1469 (98.8) | – |  |
419 | 7464 | T | 7357 (98.6) | C | 98 (1.3) | 1436 | T | 886 (61.7) | C | 550 (38.3) |
462 | 494 | A | 466 (94.3) | T | 27 (5.5) | 427 | T | 372 (87.1) | A | 54 (12.6) |
480 | 2302 | C | 2250 (97.7) | T | 48 (2.1) | 797 | T | 566 (71.0) | C | 231 (29.0) |
486 | 7114 | C | 6972 (98.0) | T | 137 (1.9) | 1489 | T | 1252 (84.1) | C | 233 (15.6) |
523 | 8630 | T | 8559 (99.2) | G | 59 (0.7) | 1376 | T | 1035 (75.2) | G | 338 (24.6) |
616 | 316 | T | 294 (93.0) | C | 21 (6.6) | 370 | C | 220 (59.5) | T | 149 (40.3) |
618 | 316 | C | 281 (88.9) | T | 35 (11.1) | 381 | T | 359 (94.2) | – |  |
645 | 1210 | C | 1172 (96.9) | T | 37 (3.1) | 351 | T | 286 (81.5) | C | 64 (18.2) |
648 | 1440 | T | 1386 (96.2) | C | 51 (3.5) | 337 | C | 331 (98.2) | – |  |
690 | 3415 | C | 3398 (99.5) | A | 17 (0.5) | 64 | A | 61 (95.3) | – |  |