Fig. 6

The hnRNPA1 PrLD is affected by genetic, post-transcriptional, and post-translational sequence variation. a Aggregation propensity scores for all hnRNPA1 splice variants, as well as all disease-associated variants, are plotted separately. Note that the N319S, D314V, and D314N mutations correspond to N267S, D262V, and D262N mutations in the short isoform, which are the more commonly referenced locations of these mutations [33]. b For comparison, similar analyses were performed for FUS. For each line in both plots, regions corresponding to FoldIndex scores > 0.0 (which are not assigned aggregation propensity scores in PAPA) are plotted as thin grey segments, whereas all regions scored by PAPA (FoldIndex< 0.0) are plotted as thick colored segments. All PTMs mapping to regions with relatively high-scoring regions (PAPA> 0.0) are indicated by vertical red lines, with line styles indicating distinct types of PTMs. For simplicity, only PTMs mapping to the longest isoform are indicated. The classical PAPA = 0.05 threshold is indicated with a dashed grey line