Preliminary investigation demonstrating the GHITM gene probably involved in apoptosis and growth of the golden apple snail (Pomacea canaliculata)

Table 1 Primers used in gene cloning and RT-qPCR

Primer	Sequences (5′-3′)	Purpose
GHITM-F	ACTTCCTTTTGCTGCTTCC	Conventional PCR
GHITM-R	GGAGCACTTGTCTACTATGGGT	Conventional PCR
5′- GHITM-R	GATTACGCCAAGCTTCCTGTCTTTGCTCAACGCCCTTAT	RACE
3′- GHITM-F	GATTACGCCAAGCTTCCTGCGCTTAACTTCTTCTGACCAC
Long primer	CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT
Short primer	CTAATACGACTCACTATAGGGC
qGHITM-F	CGCTGTTTCTCCCTACCAT	qRT-PCR
qGHITM-R	AAGCCAGGAATTTCTCATC
q18S RNA-F	AATACATGCAAACCAGCTCC
q18S RNA-R	ATTTTTCGTCACTACCTCCC

ISSN: 1471-2164