Fig. 2

Analysis of CNCC end structure determination sensitivity. a CNCC analysis of DSBs generated by AsiSI limited digestion (top). When all AsiSI cut sites (0.03% of total reads) are masked, CNCC at − 3 shift (marked by red rectangles) drops to the average level (bottom). b The CNCC signal at − 3 shift (blue) with an increasing percentage of cut sites masked shows a concordant decrease in the CNCC signal at − 3 shift, while the corresponding shuffled control remains constant (gray). Box represents 25 and 75%, and median is marked with the bar within the box. Whiskers depict 5 and 95%, and outliers are marked with ellipses