Fig. 5

Target sequences prediction and RLM 5′-RACE validation of JcSPLs cleaved by the miR156 and expression of miR156. a Target sequences of 10 JcSPLs through psRNATarget prediction. Left are the multiple alignment of miR156 complementary sequences with their targets. Right are the gene structure of corresponding JcSPLs. Blue box indicates SBP site. Purple box indicates the miR156 target site. b RLM-5′-RACE validation of JcSPL9 cleavage sites by JcmiR156a. The red box indicates PCR products for RLM 5′-RACE. The arrow indicates cleavage sites verified by RLM 5′-RACE with the sequencing frequency (sequencing reads/total sequenced clones) of cloned PCR products. c Relative expression of miR156a and JcSPL3. The expression levels at 1-month-old plants were set as 1.0. The value was shown as the mean ± the standard deviation (n = 3)