Genome re-sequencing and reannotation of the Escherichia coli ER2566 strain and transcriptome sequencing under overexpression conditions

Table 2 Statistical analysis of RNA-seq data

Sample	Run	Raw sequences reads	Unidentified reads^a	HPV16L1 reads^b	E. coli reads^b
B37	1	22,781,394 (100%)	250,596 (1%)	0 (0%)	22,334,273 (99%)
	2	22,763,848 (100%)	318,694 (1%)	0 (0%)	22,312,577 (99%)
	3	29,884,996 (100%)	597,700 (2%)	0 (0%)	29,276,302 (98%)
Y37	1	28,292,578 (100%)	4,526,812 (16%)	12,731,660 (45%)	11,034,106 (39%)
	2	25,214,878 (100%)	4,286,528 (17%)	11,346,696 (45%)	9,581,654 (38%)
	3	22,521,416 (100%)	3,378,212 (15%)	10,134,638 (45%)	9,008,566 (40%)

^a: Three biological replicates of each samples were analyzed. Unidentified RNA-seq reads could include unidentified nucleotides (Ns), short reads, low quality reads, unaligned recombinant protein reads and a larger number of mRNA from plasmids. ^b: HPV16L1 reads and E. coli reads respectively represent Human papillomavirus and Escherichia coli organism reads

ISSN: 1471-2164