Fig. 5

Balneolaeota LexA regulon reconstruction. a LexA-binding motif of the Balneolaeota generated by MEME. b CGB-generated hierarchical heatmap of posterior probability of regulation for orthologous groups. Each row represents an orthologous group, sorted by average posterior probability of regulation. Cells are colored from green (regulation) to red (no regulation), with blue denoting absence of ortholog. The gene locus tag and operon number are displayed for each ortholog. c Sites identified by CGB in the promoter region of genes with orthologs in at least six of the seven species and with average posterior probability greater than 0.5. EMSAs showing binding of purified Balneola vulgaris LexA to the promoters fragments of predicted regulated genes containing these sites. The Burkholderia cepacia recA promoter is used as negative control. Plus and minus signs indicate presence or absence of LexA in each lane