Fig. 3

HDAC3 knockdown in the last instar larvae of T. castaneum affects the expression of genes involved in JH action and response. A The knockdown of HDAC3 in newly molted last instar larvae caused an upregulation of genes involved in JH action (SRC, CBP) and JH response (Kr-h1, 4EBP, G13402). Newly molted last instar larvae were injected with dsHDAC3 or dsmalE. Total RNA was extracted at 12 h after treatment, and the mRNA levels of JH-response genes (Kr-h1, 4EBP) genes involved in JH action (Met, SRC, CBP), HSP90 and Actin were quantified. The mean ± SE (n = 4) are shown. The data were analyzed using analysis of variance, each pair student’s t-test. Mean values with the same letter are not significantly different from each other. B The knockdown of HDAC3 in pupae caused an upregulation of JH response genes (Kr-h1, 4EBP, G13402). 72 h-old last instar larvae were injected with dsHDAC3 or dsmalE. Total RNA was extracted on the fifth day after injection was used to determine relative mRNA levels of SRC, CBP, Kr-h1, 4EBP, G13402, HSP90, and Actin