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Table 2 Evaluation of the raw yield and quality of TruDrop libraries when sequenced on the NovaSeq

From: Dual indexed library design enables compatibility of in-Drop single-cell RNA-sequencing with exAMP chemistry sequencing platforms

Library Sequencer i7 i5 Targeted inDrop Read Depth Observed inDrop Reads Average % of the lane Percent perfect index reads Mean transcript Quality Score Mean Barcodes and UMI quality score
TruDrop Mouse 4 NovaSeq 6000 CCGCGGTT AGCGCTAG 50,000,000 53,655,662 0.64% 96.99% 35.57 36.22
TruDrop Mouse 5 NovaSeq 6000 TTATAACC GATATCGA 50,000,000 44,554,464 0.53% 94.13% 35.53 36.19
V2 Mouse 2 + 15% illumina PhiX NextSeq GATATCGA 65,000,000 57,847,546 37.68% 91.72% 33.06 33.02
V2 Mouse 3 + 15% illumina PhiX NextSeq GCCAAT 65,000,000 64,673,114 42.14% 92.64% 33.12 33.14
V2 Mouse 4 + 99% Illumina PhiX NovaSeq 6000 CTTGTA 50,000,000 10,985,817 0.09%
V2 Mouse 5 + 99% Illumina PhiX NovaSeq 6000 GTGAAA 50,000,000 10,144,573 0.08%
V2 Mouse 4 + 0% Illumina PhiX NextSeq CTTGTA 100,000,000 97,872,275 24% 31.89 28.67
V2 Mouse 5 + 0% Illumina PhiX NextSeq GTGAAA 100,000,000 92,742,820 23% 32.03 28.09