Table 2 Comparison of six M. purpureus, M. ruber, and M. pilosus strains based on Monascus azaphilone pigment polyketide synthase gene

30

57

88

108

117

123

161

216

219

288

291

321

369

376

392

394

423

442

447

466

471

478

483

516

578

589

606

627

648

663

685

703

748

774

802

820

834

860

939

966

1022

1074

1085

1112

1152

1172

1180

1186

1188

1193

M .pilosus 4520

A

A

A

T

T

G

G

G

A

T

T

A

G

C

G

A

T

A

A

A

A

A

T

C

C

T

A

A

C

T

A

A

T

T

T

C

C

T

T

A

A

T

C

A

A

T

A

C

T

T

M .pilosus 4520

A

A

A

T

T

G

G

G

A

T

T

A

G

C

G

A

T

A

A

A

A

A

T

C

C

T

A

A

C

T

A

A

T

T

T

C

C

T

T

A

A

T

C

A

A

T

A

C

T

T

M. ruber NRLP

A

A

A

T

T

G

G

G

A

T

T

A

G

C

G

A

T

A

A

A

A

A

T

C

C

T

A

A

C

T

A

A

T

T

T

C

C

T

T

A

A

T

C

A

A

T

A

C

T

T

M. ruber 4483

A

A

A

T

T

G

G

G

A

T

T

A

G

C

G

A

T

A

A

A

A

A

T

C

C

T

A

A

C

T

A

A

T

T

T

C

C

T

T

A

A

T

C

A

A

T

A

C

T

T

M. ruber JF83291.6

C

G

G

C

C

C

A

A

G

C

C

G

A

G

A

G

C

G

G

C

G

G

C

T

T

G

G

G

T

C

G

G

C

G

C

C

A

C

C

G

G

C

T

G

G

C

G

T

C

C

M. purpureus v1_0

C

G

G

C

C

C

A

A

G

C

C

G

A

G

A

G

C

G

G

C

G

G

C

T

T

G

G

G

T

C

G

G

C

G

C

A

A

C

C

G

G

C

T

G

G

C

G

T

C

C

M. purpureus 4478

C

G

G

C

C

C

A

A

G

C

C

G

A

G

A

G

C

G

G

C

G

G

C

T

T

G

G

G

T

C

G

G

C

G

C

A

A

C

C

G

G

C

T

G

G

C

G

T

C

C

1204

1209

1235

1236

1241

1244

1274

1297

1305

1307

1318

1349

1352

1371

1376

1385

1391

1394

1395

1432

1487

1508

1517

1541

1601

1631

1658

1742

1793

1827

1865

1937

1973

1981

2030

2141

2187

2202

2219

2267

2273

2291

2375

2378

2405

2465

2483

2510

2532

2655

M .pilosus 4520

T

A

G

A

T

T

T

A

A

G

A

A

G

G

T

T

A

T

A

T

C

G

C

C

T

T

A

T

T

C

T

A

A

A

T

A

G

T

G

T

A

A

G

C

T

T

T

G

C

T

M .pilosus 4520

T

A

G

A

T

T

T

A

A

G

A

A

G

G

T

T

A

T

A

T

C

G

C

C

T

T

A

T

T

C

T

A

A

A

T

A

G

T

G

T

A

A

G

C

T

T

T

G

C

T

M. ruber NRLP

T

A

G

A

T

T

T

A

A

G

A

A

G

G

T

T

A

T

A

T

C

G

C

C

T

T

A

T

T

C

T

A

A

A

T

A

G

T

G

T

A

A

G

C

T

T

T

G

C

T

M. ruber 4483

T

A

G

A

T

T

T

A

A

G

A

A

G

G

T

T

A

T

A

T

C

G

C

C

T

T

A

T

T

C

T

A

A

A

T

A

G

T

G

T

A

A

G

C

T

T

T

G

C

T

M. ruber JF83291.6

C

G

C

G

C

C

G

G

G

C

G

G

A

A

C

C

G

A

C

C

T

A

T

T

C

G

G

C

C

A

C

T

G

G

C

C

A

C

A

G

G

G

A

T

G

C

C

C

T

G

M. purpureus v1_0

C

G

C

G

C

C

G

G

G

C

G

G

A

A

C

C

G

A

C

C

T

A

T

T

C

G

G

C

C

A

C

T

G

G

C

C

A

C

A

G

G

G

A

T

G

C

C

C

T

G

M. purpureus 4478

C

G

C

G

C

C

G

G

G

C

G

G

A

A

C

C

G

A

C

C

T

A

T

T

C

G

G

C

C

A

C

T

G

G

C

C

A

C

A

G

G

G

A

T

G

C

C

C

T

G

2681

2699

2708

2714

2720

2723

2747

2819

2867

2888

2929

2930

2933

2960

2970

2999

3086

3089

3178

3201

3264

3265

3266

3332

3360

3368

3381

3386

3457

3458

3483

3486

3531

3551

3602

3624

3626

3633

3650

3657

3671

3677

3688

3722

3764

3798

3800

3848

3907

3918

M .pilosus 4520

C

T

C

T

T

A

A

T

T

T

T

T

T

G

A

A

A

G

A

A

-

-

-

A

A

T

T

T

A

A

A

C

G

G

A

G

T

C

A

G

T

T

T

T

C

T

C

G

T

G

M .pilosus 4520

C

T

C

T

T

A

A

T

T

T

T

T

T

G

A

A

A

G

A

A

-

-

-

A

A

T

T

T

A

A

A

C

G

G

A

G

T

C

A

G

T

T

T

T

C

T

C

G

T

G

M. ruber NRLP

C

T

C

T

T

A

A

T

T

T

T

T

T

G

A

A

A

G

A

A

-

-

-

A

A

T

T

T

A

A

A

C

G

G

A

G

T

C

A

G

T

T

T

T

C

T

C

G

T

G

M. ruber 4483

C

T

C

T

T

A

A

T

T

T

T

T

T

G

A

A

A

G

A

A

-

-

-

A

A

T

T

T

A

A

A

C

G

G

A

G

T

C

A

G

T

T

T

T

C

T

C

G

T

G

M. ruber JF83291.6

T

C

T

C

C

G

G

C

C

C

C

C

C

T

T

G

C

A

C

G

G

A

C

G

G

C

G

C

G

G

G

T

A

A

G

A

C

T

C

A

C

G

C

C

T

C

T

A

C

A

M. purpureus v1_0

T

C

T

C

C

G

G

C

C

C

C

C

C

T

T

G

C

A

C

G

G

A

C

G

G

C

G

C

G

G

G

T

A

A

G

A

C

T

C

A

C

G

C

C

T

C

T

A

C

A

M. purpureus 4478

T

C

T

C

C

G

G

C

C

C

C

C

C

T

T

G

C

A

C

G

G

A

C

G

G

C

G

C

G

G

G

T

A

A

G

A

C

T

C

A

C

G

C

C

T

C

T

A

C

A

3919

3936

3953

4025

4058

4088

4151

4169

4248

4310

4373

4425

4481

4484

4592

4660

4670

4673

4775

4785

4808

4912

4927

4937

4958

4974

4985

4987

5021

5035

5036

5182

5194

5198

5230

5234

5276

5281

5291

5292

5293

5294

5301

5302

5363

5417

5442

5448

5471

5489

M .pilosus 4520

T

A

A

C

T

C

A

A

T

G

T

A

A

T

A

T

C

T

A

A

T

T

G

T

A

A

T

C

C

C

C

T

A

G

T

T

T

T

-

-

-

G

G

C

C

T

A

A

A

M .pilosus 4520

T

A

A

C

T

C

A

A

T

G

T

A

A

T

A

T

C

T

A

A

T

T

G

T

A

A

T

C

C

C

C

T

A

G

T

T

T

T

-

-

-

G

G

C

C

T

A

A

A

M. ruber NRLP

T

A

A

C

T

C

A

A

T

G

T

A

A

T

A

T

C

T

A

A

T

T

G

T

A

A

T

C

C

C

C

T

A

G

T

T

T

T

-

-

-

G

G

C

C

T

A

A

A

M. ruber 4483

T

A

A

C

T

C

A

A

T

G

T

A

A

T

A

T

C

T

A

A

T

T

G

T

A

A

T

C

C

C

C

T

A

G

T

T

T

T

-

-

-

G

G

C

C

T

A

A

A

M. ruber JF83291.6

C

G

G

T

C

G

G

T

C

C

C

T

G

C

T

C

T

C

C

C

C

C

A

G

G

G

C

T

T

T

T

G

G

A

C

C

G

A

G

A

G

A

A

T

T

G

G

G

G

M. purpureus v1_0

C

G

G

T

C

G

G

T

C

C

C

T

G

C

T

C

T

C

C

C

C

C

A

G

G

G

C

T

T

T

T

G

G

A

C

C

G

A

G

A

G

A

A

T

T

G

G

G

G

M. purpureus 4478

C

G

G

T

C

G

G

T

C

C

C

T

G

C

T

C

T

C

C

C

C

C

A

G

G

G

C

T

T

T

T

G

G

A

C

C

G

A

G

A

G

A

A

T

T

G

G

G

G

5492

5495

5500

5501

5527

5552

5568

5599

5615

5618

5634

5674

5714

5726

5780

5795

5807

5819

5882

5906

5987

6021

6028

6041

6149

6167

6173

6200

5021

6236

6248

6329

6347

6383

6389

6521

6569

6572

6596

6641

6759

6914

6928

7002

7006

7007

7008

7019

7024

7082

M .pilosus 4520

C

A

T

A

C

T

A

T

C

C

G

G

C

A

T

C

C

A

G

T

A

T

T

C

T

A

T

A

C

T

T

T

A

A

T

C

C

G

T

G

T

T

T

A

C

A

T

T

G

C

M .pilosus 4520

C

A

T

A

C

T

A

T

C

C

G

G

C

A

T

C

C

A

G

T

A

T

T

C

T

A

T

A

C

T

T

T

A

A

T

C

C

G

T

G

T

T

T

A

C

A

T

T

G

C

M. ruber NRLP

C

A

T

A

C

T

A

T

C

C

G

G

C

A

T

C

C

A

G

T

A

T

T

C

T

A

T

A

C

T

T

T

A

A

T

C

C

G

T

G

T

T

T

A

C

A

T

T

G

C

M. ruber 4483

C

A

T

A

C

T

A

T

C

C

G

G

C

A

T

C

C

A

G

T

A

T

T

C

T

A

T

A

C

T

T

T

A

A

T

C

C

G

T

G

T

T

T

A

C

A

T

T

G

C

M. ruber JF83291.6

T

G

G

G

T

C

G

C

G

T

A

C

G

G

A

T

T

G

A

G

G

C

C

T

A

G

C

G

T

C

C

C

C

G

C

T

T

C

C

C

C

C

C

G

-

-

-

C

A

T

M. purpureus v1_0

T

G

G

G

T

C

G

C

G

T

A

C

G

G

A

T

T

G

A

G

G

C

C

T

A

G

C

G

T

C

C

C

C

G

C

T

T

C

C

C

C

C

C

G

-

-

-

C

A

T

M. purpureus 4478

T

G

G

G

T

C

G

C

G

T

A

C

G

G

A

T

T

G

A

G

G

C

C

T

A

G

C

G

T

C

C

C

C

G

C

T

T

C

C

C

C

C

C

G

-

-

-

C

A

T

7189

7191

7241

7270

7316

7325

7343

7397

7415

7421

7445

7457

7556

7583

7631

7676

7692

7769

7853

7874

7976

8043

8044

8083

8105

8107

M .pilosus 4520

A

A

G

A

C

T

C

A

T

C

T

A

T

A

G

G

A

C

C

T

T

G

T

T

A

A

M .pilosus 4520

A

A

G

A

C

T

C

A

T

C

T

A

T

A

G

G

A

C

C

T

T

G

T

T

A

A

M. ruber NRLP

A

A

G

A

C

T

C

A

T

C

T

A

T

A

G

G

A

C

C

T

T

G

T

T

A

A

M. ruber 4483

A

A

G

A

C

T

C

A

T

C

T

A

T

A

G

G

A

C

C

T

T

G

T

T

A

A

M. ruber JF83291.6

G

C

C

G

T

C

T

C

C

T

C

G

C

G

A

A

G

T

G

C

G

C

C

G

G

G

M. purpureus v1_0

G

C

C

G

T

C

T

C

C

T

C

G

C

G

A

A

G

T

G

C

G

C

C

G

G

G

M. purpureus 4478

G

C

C

G

T

C

T

C

C

T

C

G

C

G

A

A

G

T

G

C

G

C

C

G

G

G