Identification and distribution of gene clusters required for synthesis of sphingolipid metabolism inhibitors in diverse species of the filamentous fungus Fusarium

Table 2 Examples of putative horizontal gene transfer events of SAM clusters among species of Fusarium inferred initially by NOTUNG reconciliation analysis and supported (+) or not supported (−) by constraint analysis using Shimodaira-Hasegawa and Approximately Unbiased (SH-AU) tests and by synonymous site divergence analysis

Cluster	Potential Donor	Potential Recipient	NOTUNG	SH-AU^a	Synonymous Site Divergence^b
SAM1	F. hostae	F. austroamericanum-F. brasilicum-F. cortaderia	+	+	+
SAM1	F. hostae	Fusarium sp. 66182	+	nt	+
SAM1	F. bulbicola	F. oxysporum	+	+	+
SAM3	F. burgessii	FIESC18-FIESC24-F. kyushuense	+	+	+
SAM3	F. denticulatum	F. begonia	+	nt	+
SAM4	F. pseudonygamai	F. phyllophilum	+	+	+
SAM4	F. newnesense	F. acuminatum	+	nt	+
SAM4	F. subglutinans	F. circinatum	+	–	+
SAM5	F. denticulatum-F. pseudcircinatum- F. udum	F. mangiferae	+	+	+
SAM5	F. newnesense	F. concolor	+	+	+
SAM5	F. tupiense	F. mexicanum	+	–	–

^a Results of the SH-AU tests were considered to be consistent with horizontal transfer only when P values from both tests were < 0.01. nt indicates that, given the tree topology in question, it was not possible to test the branch conflict indicative of the transfer event
^b Results of the d_S analysis were considered to be consistent with horizontal transfer when, for a given pairwise combination of taxa, the d_S value for SAM genes was less than d_S for housekeeping genes

ISSN: 1471-2164