Fig. 4

Comparison of 8q for U2OS, UMUC3, and LNCaP. a The hg38 subtelomere reference sequence for 8q is shown as a light blue bar; the dark blue vertical ticks within this bar indicate in silico Nt.BspQI nick-label sites. The location of the telomere is shown with a red “T”. Representative examples of raw images of single molecules that were imaged are shown below the reference for each of the cell lines. The DNA backbone is blue, and both the labeled subtelomeric Nt.BspQI sites and the telomere (TTAGGG) n tracts are green. The top U2OS molecule has an end telomere with a (TTAGGG) n tract, the second has undergone recombination with retention of an ITS, telomere, the third has undergone recombination without retention of an ITS, and the bottom molecule has an end telomere loss. The Saos-2 molecules retain their ITS., but SK-MEL-2 has only one ITS. b Comparison of U2OS, Saos-2 and SK-MEL-2 end telomeres and internal telomere length with UMUC3 and LNCaP end telomere length. Each single molecule telomere length measurement is represented by a dot and the average telomere length for each chromosome arm is shown as a horizontal line. UMUC3 and LNCap don’t have any ITS due to recombination