Precise annotation of tick mitochondrial genomes reveals multiple copy number variation of short tandem repeats and one transposon-like element

Table 1 PCR primers for the Dermacentor mt genomes

Forward primer	Reverse primer	Segment	Size(bp)
TCAGTCATTTTACCGCGATGA	GCTCAAATTCCATTCTCTGC	L1	9580
AGCTGTTACTAACGTTGAGG	AGGATGTTGATGGATCGAAA	L2	8156
GCTAKTGGGTTCATACCCCAA	CGACCTCGATGTTGGATTAGGA	L3	7155
CCAACCTGATTCWCATCGGTCT	TCATCGCGGTAAAATGACTGA	L4	9187
TGCTGCTGGCACAAATTTAGC	CAAGATGACCCTAAATTCAGGCA	CR1	483
GGAGCTATACCAATTGAATATCCC	TTGGGGTATGAACCCAATAGC	CR2	645
TGCATTCAGTTTCGGCCTGA	CCGGCTGTCTCATCTATTGAC	R2	3616
CTATTCCGGCATAGTAAAATGCCTG	CAAGCTTATGCACCCTTTTCAATAC	R1	570

These primers were designed to amplify large segments (L1, L2, L3 and L4) and short segments (CR1, CR2, R1 and R2) in the mt genomes of the genus Dermacentor. Their PCR reaction conditions can be seen in the Methods. Based on the results using 100 individual ticks from four species, the primers for L3 and L4 were optimized to amplify more species of the genus Dermacentor than those of L1 and L2. The R2 segment spanned tRNA^Arg, tRNA^Asn, tRNA^Ser, tRNA^Glu, ND1, tRNA^Leu, 16S rRNA, tRNA^Val, 12S rRNA and CR1. The R1 segment spanned tRNA^Ile, tRNA^Gln, R1, tRNA^Phe and ND5. The segment sizes were estimated using the D. silvarum mt genome (GenBank: MN347015)

ISSN: 1471-2164